Device
Pbad-ChiXR

Part:BBa_K564011:Design

Designed by: Anna Lewinska   Group: iGEM11_DTU_Denmark   (2011-09-20)


ara-->chiXR (sRNA) device


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

There are no design considerations.


Source

Arabinose promoter was obtained from PCR of biobrick I0500 and ChiX was designed and ordered as a minigene. Then the two parts were fused together.

References